Annette Walther studied in Düsseldorf, Essen and London with Ida Bieler, David Takeno and Vesselin Parschkevov. At the Guildhall School for Music and Drama in London she also took chamber music classes with Sir Colin Davis and the Takacs Quartet. Further masterclasses with Michelle Auclair, Yuri Bashmet, Thomas Brandis, Jürgen Kussmaul and Charles-Andre Linale have been of lasting importance to her musical development.

Annette has been a stipendiary of the Villa Musica and the Hartmut Schuler Foundations, and in 2004 she was awarded the Artland Musikpreis.

In addition to being a founding member of the Signum Quartet, Annette regularly performs with artists such as Carolin Widmann, Nils Mönkemeyer, William Youn, David Cohen, Liza Ferschtman and Priya Mitchell at festivals including the Musikfestspielen Mecklenburg –Vorpommern, Lofoten International Chamber Music Festival, Musiktage Hitzacker and Kammermusikfest Sylt.

She is a regular guest in the Mahler Chamber Orchestra and ensemble musikfabrik, concertmaster of the Cologne Chamber Orchestra, the Belgian Chamber Orchestra and the Folkwang Chamber Orchestra. She is a member of the Geneva Camerata and Ensemble Ruhr.

At the beginning of 2016, Annette was appointed as violin lecturer at the Louis Spohr Music Academy in Kassel.